李光鹏

行政职务：内蒙古大学实验动物研究中心主任；教育部生殖生物学及生物技术重点实验室主任

学  位： 博士

导师情况：博士生导师

研究领域：1. 体细胞重编程机理；2. 早期胚胎发育分子机制；3. 动物转基因技术与分子育种。

电子邮件: gpengli@imu.edu.cn

 

学习工作经历：

1981年9月-1985年7月，山东大学生物系动物胚胎学专业，学士；

1985年7月-1987年9月，东北林业大学野生动物系，助教；

1987年9月-1990年7月，东北农业大学动物解剖与组织胚胎学专业学习，硕士；

1990年7月-1992年11月，黑龙江科学院自然资源研究所，助理研究员；

1992年12月-1996年5月，东北农业大学生物动物组织胚胎学教研室，讲师；

1995年9月-1998年12月，东北农业大学动物解剖与组织胚胎专业，博士；

1996年5月-1998年12月，东北农业大学动物组织胚胎学教研室，副教授；

1998年12月-2000年7月，中国科学院动物研究所计划生育国家重点实验室，博士后, 副教授；

2000年7月-2001年6月，美国科罗拉多州立大学动物繁殖与生物工程实验室，访问学者；

2001年6月-2001年12月，美国犹他州立大学，动物，乳品和兽医科学系，访问学者；

2002年1月-2007年10月，美国犹他州立大学，动物，乳品和兽医科学系，研究助理教授；

2007年5月- 教育部“长江学者”特聘教授，受聘于内蒙古大学实验动物研究中心。

 

荣誉及奖励：

2005年，国家自然科学奖二等奖

2007年，长江学者奖励计划特聘教授

2009年，全国优秀专业人才先进集体

2011年，内蒙古自治区“草原英才”称号

2011年，内蒙古自治区“五一”劳动奖

2012年，荣获第四届新侨创新成果交流会“中国侨界创新成果贡献奖”

2013年，入选科技部重点领域创新团队

2014年，全国先进科技工作者

2015年，第九届大北农科技奖创新奖

2016年，国家高层次人才特殊支持计划（“万人计划”）科技创新领军人才

2016年，国家技术发明二等奖

研究方向与主要学术成就：

哺乳动物卵子发生机理、体细胞重编程机理、显微操作及动物转基因技术等。首次证明初级、次级卵母细胞纺锤体互换的可行性，获得世界首例初级、次级卵母细胞纺锤体互换哺乳动物；世界首例克隆骡子的创制者；获得世界第二例胚胎克隆猪；成功克隆了牛、绵羊、山羊、猪、兔、小鼠；建立高效转基因克隆奶牛、肉牛、绵羊等技术体系；将克隆牛、羊技术成功实现了产业转化；在大熊猫、藏羚羊、普氏原羚、猪、小鼠等异种克隆机理等方面进行系统性的研究。

 

课题主持：

1.国家科技重大专项课题：“高产优质转基因肉牛新品种培育”，总负责人

2.国家“863”项目：“动物转基因删除技术与育种应用研究” ，总负责人

3.国家“863”项目子课题：“转录因子介入的转基因克隆动物研究”，负责人

4.国家“973”项目子课题：“家畜克隆与转基因克隆激励研究”，负责人

5.国家自然科学基金课题：“多不饱和脂肪酸去饱和酶基因（fat-1）转基因绵羊外源基因的表达调控研究”，负责人

6.内蒙古自治区科技重大项目：“高产转基因优质肉羊新品种培育”，总负责人

7.内蒙古自治区重大项目：“内蒙古草原生态畜牧产业研究院”，总负责人

 

社会兼职：

1、中国生物技术学会动物生物技术学分会副理事长

2、中国动物学会细胞与分子生物学分会副理事长

3、中国动物学会生殖生物学分会理事

 

目前，共发表论文180多篇 , 其中SCI论文80余篇；主编及参编学术著作7部，参与翻译专著1部。

著作：

　　1.《发育生物学原理与胚胎工程》，主编，黑龙江科学技术出版社 1995；

　　2.《受精生物学--受精机制与生殖工程》，副主编，科学出版社2001；

　　3.《哺乳动物胚胎学》，参编，科学出版社，2001；

　　4. White KL, Woods GL, Vanderwall DK, Li GP, Bunch TD. Cloning the Equine. In: Epigenetic Risks of Cloning. Edited by Akio Inui, 2006. pp 59-70. CRC Press, Taylor & Francis Group, Boca Raton, Florida, USA；

5.《小鼠胚胎操纵实验指南》，参译，化学工业出版社，2006。

6.《发育与进化》，参编，科学出版社，2011；

7.《动物解剖学、组织学与生殖生物学简明教程》，主编，内蒙古大学出版社，2016；

8.《生物技术概论》，主编，内蒙古大学出版社，2017.

 

代表性论文：

    1. Zuo, Y., Su, G., Wang, S., Yang, L., Liao, M., Wei, Z., Bai, C. and Li, G. (2016) Exploring timing activation of functional pathway based on differential co-expression analysis in preimplantation embryogenesis. Oncotarget, 7, 74120-74131.

    2. Yang, L., Song, L.S., Liu, X.F., Xia, Q., Bai, L.G., Gao, L., Gao, G.Q., Wang, Y., Wei, Z.Y., Bai, C.L. et al. (2016) The Maternal Effect Genes UTX and JMJD3 Play Contrasting Roles in Mus musculus Preimplantation Embryo Development. Sci Rep, 6, 26711.

    3. Wu, X., Song, M., Yang, X., Liu, X., Liu, K., Jiao, C., Wang, J., Bai, C., Su, G., Liu, X. et al. (2016) Establishment of bovine embryonic stem cells after knockdown of CDX2. Sci Rep, 6, 28343.

    4. Zuo, Y.C., Su, W.X., Zhang, S.H., Wang, S.S., Wu, C.Y., Yang, L. and Li, G.P. (2015) Discrimination of membrane transporter protein types using K-nearest neighbor method derived from the similarity distance of total diversity measure. Mol Biosyst, 11, 950-957.

    5. Wang, Y., Lv, Y., Wang, L., Gong, C., Sun, J., Chen, X., Chen, Y., Yang, L., Zhang, Y., Yang, X. et al. (2015) MicroRNAome in decidua: a new approach to assess the maintenance of pregnancy. Fertil Steril, 103, 980-989 e986.

    6. Zuo, Y., Gao, Y., Su, G., Bai, C., Wei, Z., Liu, K., Li, Q., Bou, S. and Li, G. (2014) Irregular transcriptome reprogramming probably causes thec developmental failure of embryos produced by interspecies somatic cell nuclear transfer between the Przewalski's gazelle and the bovine. BMC Genomics, 15, 1113.

    7. Liu, Y., Wei, Z., Huang, Y., Bai, C., Zan, L. and Li, G. (2014) Cyclopamine did not affect mouse oocyte maturation in vitro but decreased early embryonic development. Anim Sci J, 85, 840-847.

    8. Wu, J., Liao, M., Zhu, H., Kang, K., Mu, H., Song, W., Niu, Z., He, X., Bai, C., Li, G. et al. (2014) CD49f-positive testicular cells in Saanen dairy goat were identified as spermatogonia-like cells by miRNA profiling analysis. J Cell Biochem, 115, 1712-1723.

    9. Yin, F.X., Li, G.P., Bai, C.L., Liu, Y., Wei, Z.Y., Liang, C.G., Bunch, T.D. and Zan, L.S. (2013) SGO1 maintains bovine meiotic and mitotic centromeric cohesions of sister chromatids and directly affects embryo development. PLoS One, 8, e73636.

   10. Liu, H., Yin, F.X., Bai, C.L., Shen, Q.Y., Wei, Z.Y., Li, X.X., Liang, H., Bou, S. and Li, G.P. (2013) TFIIB co-localizes and interacts with alpha-tubulin during oocyte meiosis in the mouse and depletion of TFIIB causes arrest of subsequent embryo development. PLoS One, 8, e80039.

   11. Deng, S., Li, G., Zhang, J., Zhang, X., Cui, M., Guo, Y., Liu, G., Li, G., Feng, J. and Lian, Z. (2013) Transgenic cloned sheep overexpressing ovine toll-like receptor 4. Theriogenology, 80, 50-57.

   12. Yang, F., Wei, Z., Ding, X., Liu, X., Ge, X., Song, G., Li, G. and Guo, H. (2013) Upregulation of triglyceride synthesis in skeletal muscle overexpressing DGAT1. Lipids Health Dis, 12, 63.

   13. Duan, B., Cheng, L., Gao, Y., Yin, F.X., Su, G.H., Shen, Q.Y., Liu, K., Hu, X., Liu, X. and Li, G.P. (2012) Silencing of fat-1 transgene expression in sheep may result from hypermethylation of its driven cytomegalovirus (CMV) promoter. Theriogenology, 78, 793-802.

   14. Wu, X., Ouyang, H., Duan, B., Pang, D., Zhang, L., Yuan, T., Xue, L., Ni, D., Cheng, L., Dong, S. et al. (2012) Production of cloned transgenic cow expressing omega-3 fatty acids. Transgenic Res,21, 537-543.

   15. Xu, C., Lu, X., Chen, E.Z., He, Z., Uyunbilig, B., Li, G., Ma, Y., Hui, L., Xie, B., Gao, Y. et al. (2012) Genome-wide roles of Foxa2 in directing liver specification. J Mol Cell Biol, 4, 420-422.

   16. Wang, H., Wu, J., Liu, X., He, H., Ding, F., Yang, H., Cheng, L., Liu, W., Zhong, J., Dai, Y. et al. (2012) Identification of short hairpin RNA targeting foot-and-mouth disease virus with transgenic bovine fetal epithelium cells. PLoS One, 7, e42356.

   17. He, Z.Y., Deng, L., Li, Y.F., Xiang, D., Hu, J.K., Chen, Y.X., Wang, M.J., Chen, F., Liu, C.C., Li, W.L. et al. (2012) Murine embryonic stem cell-derived hepatocytes correct metabolic liver disease after serial liver repopulation. Int J Biochem Cell Biol, 44, 648-658.

   18. Xue, L., Cheng, L., Su, G., Kang, F., Wu, X., Bai, C., Zhang, L. and Li, G.P. (2011) Nuclear transfer procedures in the ovine can induce early embryo fragmentation and compromise cloned embryo development. Anim Reprod Sci, 126, 179-186.

   19. Meng, Q., Wu, X., Bunch, T.D., White, K., Sessions, B.R., Davies, C.J., Rickords, L. and Li, G.P. (2011) Enucleation of demecolcine-treated bovine oocytes in cytochalasin-free medium: mechanism investigation and practical improvement. Cell Reprogram, 13, 411-418.

   20. Bai, C., Liu, H., Liu, Y., Wu, X., Cheng, L., Bou, S. and Li, G.P. (2011) Diploid oocyte formation and tetraploid embryo development induced by cytochalasin B in bovine. Cell Reprogram, 13, 37-45.

   21. Chen, F., Wang, H., He, H., Song, L., Wu, J., Gao, Y., Liu, X., He, C., Yang, H., Chen, L. et al. (2011) Short hairpin RNA-mediated silencing of bovine rotavirus NSP4 gene prevents diarrhoea in suckling mice. J Gen Virol, 92, 945-951.

   22. Wu, X., Li, Y., Xue, L., Wang, L., Yue, Y., Li, K., Bou, S., Li, G.P. and Yu, H. (2011) Multiple histone site epigenetic modifications in nuclear transfer and in vitro fertilized bovine embryos. Zygote,19, 31-45.

   23. Aston, K.I., Li, G.P., Hicks, B.A., Sessions, B.R., Davis, A.P., Rickords, L.F., Stevens, J.R. and White, K.L. (2010) Abnormal levels of transcript abundance of developmentally important genes in various stages of preimplantation bovine somatic cell nuclear transfer embryos. Cell Reprogram, 12, 23-32.

   24. Li, G.P., Yang, S., Liu, Y., Sessions, B.R., White, K.L. and Bunch, T.D. (2009) Nicotine combined with okadaic acid or taxol adversely affects bovine oocyte maturation and subsequent embryo development. Fertil Steril, 92, 798-805.

   25. Aston, K.I., Li, G.P., Hicks, B.A., Winger, Q.A. and White, K.L. (2009) Genetic reprogramming of transcription factor ap-2gamma in bovine somatic cell nuclear transfer preimplantation embryos and placentomes. Cloning Stem Cells, 11, 177-186.

   26. Li, G.P., White, K.L., Aston, K.I., Bunch, T.D., Hicks, B., Liu, Y. and Sessions, B.R. (2009) Colcemid-treatment of heifer oocytes enhances nuclear transfer embryonic development, establishment of pregnancy and development to term. Mol Reprod Dev, 76, 620-628.

   27. Aston, K.I., Li, G.P., Hicks, B.A., Sessions, B.R., Davis, A.P., Winger, Q.A., Rickords, L.F., Stevens, J.R. and White, K.L. (2009) Global gene expression analysis of bovine somatic cell nuclear transfer blastocysts and cotyledons. Mol Reprod Dev, 76, 471-482.

   28. Liu, Y., Li, G.P., Sessions, B.R., Rickords, L.F., White, K.L. and Bunch, T.D. (2008) Nicotine induces multinuclear formation and causes aberrant embryonic development in bovine. Mol Reprod Dev, 75, 801-809.

   29. Liu, Y., Li, G.P., Rickords, L.F., White, K.L., Sessions, B.R., Aston, K.I. and Bunch, T.D. (2008) Effect of nicotine on in vitro maturation of bovine oocytes. Anim Reprod Sci, 103, 13-24.

   30. Liu, Y., Li, G.P., White, K.L., Rickords, L.F., Sessions, B.R., Aston, K.I. and Bunch, T.D. (2007) Nicotine alters bovine oocyte meiosis and affects subsequent embryonic development. Mol Reprod Dev,74, 1473-1482.

   31. Aston, K.I., Li, G.P., Hicks, B.A., Sessions, B.R., Pate, B.J., Hammon, D.S., Bunch, T.D. and White, K.L. (2006) The developmental competence of bovine nuclear transfer embryos derived from cow versus heifer cytoplasts. Anim Reprod Sci, 95, 234-243.

   32. Li, G.P., Bunch, T.D., White, K.L., Rickords, L., Liu, Y. and Sessions, B.R. (2006) Denuding and centrifugation of maturing bovine oocytes alters oocyte spindle integrity and the ability of cytoplasm to support parthenogenetic and nuclear transfer embryo development. Mol Reprod Dev, 73, 446-451.

   33. Aston, K.I., Li, G.P., Hicks, B.A., Sessions, B.R., Pate, B.J., Hammon, D., Bunch, T.D. and White, K.L. (2006) Effect of the time interval between fusion and activation on nuclear state and development in vitro and in vivo of bovine somatic cell nuclear transfer embryos. Reproduction,131, 45-51.

   34. Li, G.P., Liu, Y., Bunch, T.D., White, K.L. and Aston, K.I. (2005) Asymmetric division of spindle microtubules and microfilaments during bovine meiosis from metaphase I to metaphase III. Mol Reprod Dev, 71, 220-226.

   35. Li, G.P., Liu, Y., White, K.L. and Bunch, T.D. (2005) Cytogenetic analysis of diploidy in cloned bovine embryos using an improved air-dry karyotyping method. Theriogenology, 63, 2434-2444.

   36. Li, G.P., White, K.L. and Bunch, T.D. (2004) Review of enucleation methods and procedures used in animal cloning: state of the art.Cloning Stem Cells, 6, 5-13.

   37. Li, G.P., White, K.L., Aston, K.I., Meerdo, L.N. and Bunch, T.D. (2004) Conditioned medium increases the polyploid cell composition of bovine somatic cell nuclear-transferred blastocysts. Reproduction,127, 221-228.

   38. Woods, G.L., White, K.L., Vanderwall, D.K., Li, G.P., Aston, K.I., Bunch, T.D., Meerdo, L.N. and Pate, B.J. (2003) A mule cloned from fetal cells by nuclear transfer. Science, 301, 1063.